ELITE™ Custom Phage Display Antibody Services
Abgent offers robust custom phage display antibody production for biological research and drug discovery projects. The target gene is randomly mutated to produce a diverse library of scFv/Fab antibody fragments which are then displayed on the external surface of a filamentous phage [M13, fd, and f1 strains] as fusion proteins.
Using an iterative binding affinity-based screen called panning, a discrete number of phages that display antibodies specifically binding to the target of choice with an affinity of 10-8 to 10-9 are recovered from the phage library. Finally, the chosen antibody fragments displayed by the selected phages are identified by phage amplification, followed by DNA sequencing.
|Construction of phage display cDNA library|
|Construction of a phage display library with 10-8 to 10-9 unique members|
|Panning of the library clones by affinity chromatography|
|Screening of phage display antibody libraryand amplification (4 rounds)unique members|
|Expression and purification of selected high affinity scFv and Fab antibodies in bacterial cells|
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