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Catalog #
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Applications:
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Reactivity:
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Clone Name:
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AM3111a
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WB, E
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H
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1AT1979
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Concentration:
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Size:
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Accession:
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Isotype:
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Crude ascites
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0.1 ml
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NP_997006, Q13480
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Mouse IgG1
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Target/Specificity:
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This antibody is generated from mice immunized with a GST fusion protein encoding full length human GAB1.
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Other Names:
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GRB2-associated-binding protein 1; GRB2-associated binder 1; GAB1;
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Application Data:
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Calculated MW:
76616 Da
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(TOP) Wild-type (+/+) and Gab1-/- (-/-) cells were heat shocked (HS) at 42°C for 1 h or irradiated with UV-B light (400 J/m2) and then incubated at 37°C for 1 h. Cell lysates were prepared and subjected to immunoprecipitation (IP) with an anti-JNK2 antibody. The immunoprecipitates were resolved by SDS- PAGE and immunoblotted (IB) with anti-Gab1 antibody. Data courtesy of Dr. GS Feng at The Burnham Institute.
(BUTTOM) Rescue of the JNK pathway by expression of wild-type Gab1 in Gab1-/- cells. Gab1-/- cells were transiently transfected with 1, 2, or 4 µg of the expression construct for human Gab1 cDNA, using the GenePORTER 2 transfection reagent (Gene Therapy Systems Inc.). After incubation, the cells were irradiated with UV-B light at 400 J/m2 or left untreated. Exogenous expression of Gab1 in transfected cells was confirmed by anti-Gab1 immunoblot analysis with wild-type cells as positive control. +/+, wild-type cells; -/-, Gab1-/- cells. (Mol. Cell. Biol. 2004 Feb 15;24(4):1531-1539) |
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Product Citations:
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- Sun, Yingqing, et al. Mol. Cell. Biol. 2004 Feb 15;24(4):1531-1539. (Applications: WB)
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western Blot | 1:200~1000 |
| Immuniprecipitation | 2-5 ug |
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Format:
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Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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Order Information
List Price (USA): $235.00/0.1ml
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