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Catalog #
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Applications:
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Reactivity:
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Clone Name:
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AP1248a
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WB, IHC, E
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H
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RB0676
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Concentration:
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Size:
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Accession:
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Isotype:
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0.25 mg/ml
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0.1 mg
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NP_775298, O75928-2
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Rabbit Ig
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide unique to the C-terminal region of human PIASx1.
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Other Names:
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E3 SUMO-protein ligase PIAS2; Protein inhibitor of activated STAT2; Protein inhibitor of activated STAT x;; DAB2-interacting protein; DIP; Androgen receptor-interacting protein 3; ARIP3; PIAS-NY protein; PIAS2; PIASX; PIAS2-alpha; PIASx-alpha
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Application Data:
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Calculated MW:
63396 Da
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Western blot analysis of PIASx1 polyclonal antibody (Cat. #AP1248a) with bacterial and HL-60 cell lysates. Lane A: endogenous PIASx1 in HL-60 cells; Lane B: bacteria expressing recombinant GST-PIASx1 fusion protein. PIASx1 was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Product Citations:
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- Jones, Marius C., et al. PNAS 2006 Oct 31;103(44):16272-16277. (Applications: WB)
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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Order Information
List Price (USA): $235.00/0.1mg
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