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Bak BH3 Domain Antibody

Purified Rabbit Polyclonal Antibody (Pab)


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Catalog # Applications: Reactivity: Clone Name:
AP1301a WB, IHC, E H RB0949
Concentration: Size: Accession: Isotype:
0.25 mg/ml 0.1 mg NP_001179, Q16611 Rabbit Ig
Target/Specificity:
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region (BH3 domain) of human Bak.
Other Names:
BCL2-antagonist/killer 1; cell death inhibitor 1; apoptosis regulator BAK; Bcl-2 homologous antagonist/killer; BCL2-like 7protein
Application Data:   Calculated MW:   23411 Da
     

The anti-Bak BH3 domain Pab (Cat. #AP1301a) is used in Western blot to detect Bak in A375 cell lysate.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

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Application Notes:
The suggested dilution will be:
ELISA1:1,000
Western blotting1:100~500
Immunohistochemistry1:50~100
Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage:
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Precautions:
This product is for research use only. Not for use in diagnostic or therapeutic procedures.

 

 

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in stockList Price (USA): $235.00/0.1mg

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Abgent is the original manufacturer of this product.
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  1. BP1301a:  Blocking Peptide
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