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Catalog #
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Applications:
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Reactivity:
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Clone Name:
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AP1303a
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WB, IHC, IP, E, IF†, FC†
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H More
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RB4684
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Concentration:
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Size:
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Accession:
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Isotype:
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0.25 mg/ml
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0.1 mg
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NP_000624, P10415
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Rabbit Ig
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the BH3 domain of human Bcl2.
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Other Names:
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Apoptosis regulator Bcl-2, B-cell lymphoma protein 2
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Application Data:
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Calculated MW:
26266 Da
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Left panel, analysis of Bcl-2 BH3 domain exposure in HEK293 cells transfected with a plasmid coding for a DNA-binding domain-deleted construct of Nur77 (GFP-Nur77/dDBD) by using AP1303a antibody for immunoprecipitation (IP) and a different Bcl-2 antibody for western blot. Right panel, same analysis using AP1303a in flow cytometry. Data courtesy Dr Quan Chen, Chinese Acadamy of Sciences. |
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Bcl-2 BH3 Domain Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Product Citations:
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- Cristiano Ferlini, et al. Cancer Res. published 11 August 2009, 10.1158/0008-5472.CAN-09-0540. (E-pub)
- Xingming Deng, et al. Blood, Jan 2009; 113: 422 - 428.
- Jie Liu, et al. Cancer Res., Nov 2008; 68: 8871 - 8880. (Applications: IF)
- Jennifer Thompson, et al. J. Exp. Med., May 2008; 205: 1029 - 1036.
- Victoria Del Gaizo Moore, et al. Blood, Feb 2008; 111: 2300 - 2309.
- Lei, Xiaobo, et al. FASEB J 2006 Oct 01;20(12):2147-2149. (Applications: WB, FC)
- Deng, Xingming, et al. Mol. Cell. Biol. 2006 Jun 15;26(12):4421-4434. (Applications: WB)
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Application Notes:
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| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:10~50 |
| Immunoprecipitation | 1:100~500 |
| Flow cytometry | 1:100~500 |
† Application validated by third-party citation.
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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Order Information
List Price (USA): $235.00/0.1mg
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