primary (Immunohistochemistry & Apoptosis Antibodies) antibodies and peptides
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Bcl-2 Antibody (BH3 Domain Specific)

Purified Rabbit Polyclonal Antibody (Pab)


specificationbackgroundprotocolsprintmsds
 
Catalog # Applications: Reactivity: Clone Name:
AP1303a WB, IHC, IP, E, IF, FC H  More RB4684
Concentration: Size: Accession: Isotype:
0.25 mg/ml 0.1 mg NP_000624, P10415 Rabbit Ig
Target/Specificity:
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the BH3 domain of human Bcl2.
Other Names:
Apoptosis regulator Bcl-2, B-cell lymphoma protein 2
Application Data:   Calculated MW:   26266 Da
     
Bcl-2 Antibody (BH3 Domain Specific)

Left panel, analysis of Bcl-2 BH3 domain exposure in HEK293 cells transfected with a plasmid coding for a DNA-binding domain-deleted construct of Nur77 (GFP-Nur77/dDBD) by using AP1303a antibody for immunoprecipitation (IP) and a different Bcl-2 antibody for western blot. Right panel, same analysis using AP1303a in flow cytometry. Data courtesy Dr Quan Chen, Chinese Acadamy of Sciences.

Bcl-2 Antibody (BH3 Domain Specific)

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Bcl-2 BH3 Domain Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Product Citations:
  1. Cristiano Ferlini, et al. Cancer Res. published 11 August 2009, 10.1158/0008-5472.CAN-09-0540. (E-pub)
  2. Xingming Deng, et al. Blood, Jan 2009; 113: 422 - 428.
  3. Jie Liu, et al. Cancer Res., Nov 2008; 68: 8871 - 8880. (Applications: IF)
  4. Jennifer Thompson, et al. J. Exp. Med., May 2008; 205: 1029 - 1036.
  5. Victoria Del Gaizo Moore, et al. Blood, Feb 2008; 111: 2300 - 2309.
  6. Lei, Xiaobo, et al. FASEB J 2006 Oct 01;20(12):2147-2149. (Applications: WB, FC)
  7. Deng, Xingming, et al. Mol. Cell. Biol. 2006 Jun 15;26(12):4421-4434. (Applications: WB)

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Application Notes:
ELISA1:1,000
Western blotting1:100~500
Immunohistochemistry1:10~50
Immunoprecipitation1:100~500
Flow cytometry1:100~500

† Application validated by third-party citation.
Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage:
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Precautions:
This product is for research use only. Not for use in diagnostic or therapeutic procedures.

 

 

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in stockList Price (USA): $235.00/0.1mg

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