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Catalog #
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Applications:
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Reactivity:
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Clone Name:
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AP1312a
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WB, IHC, E,
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H, M
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RB0961
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Concentration:
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Size:
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Accession:
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Isotype:
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0.25 mg/ml
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0.1 mg
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NP_068779, Q07820
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Rabbit Ig
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Target/Specificity:
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This antibody was developed against a synthetic peptide selected from the central region (BH3 domain) of human Mcl-1.
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Other Names:
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Induced myeloid leukemia cell differentiation protein Mcl-1; Bcl-2-related protein EAT/mcl1; mcl1/EAT; MCL1
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Application Data:
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Calculated MW:
37323 Da
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Western blot analysis of anti-Mcl-1 BH3 Domain Pab (Cat. #AP1312a) in A375 cell lysate. Mcl-1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Product Citations:
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- Johnson, Amy J., et al. Blood 2006 Aug 15;108(4):1334-1338.
- Yamane, Toshiyuki, et al. PNAS 2005 Mar 01;102(9):3312-3317.
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Application Notes:
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| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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Order Information
List Price (USA): $235.00/0.1mg
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