|
Catalog #
|
Applications:
|
Reactivity:
|
Clone Name:
|
|
AP1813d
|
WB, IHC, E
|
H, C*, M*, R* More
|
RB7474
|
|
Concentration:
|
Size:
|
Accession:
|
Isotype:
|
|
0.25 mg/ml
|
0.1 mg
|
NP_006386, O95352
|
Rabbit Ig
|
|
Target/Specificity:
|
|
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide at the C-term of human APG7L.
|
|
Other Names:
|
|
Autophagy protein 7-like; APG7-like; Ubiquitin activating enzyme E1-like protein; hAGP7; APG7L; ATG7
|
|
Application Data:
|
|
Calculated MW:
77960 Da
|
|
|
|
|
Western blot analysis of APG7L (C-term) Pab (Cat. #AP1813d) TOP LEFT: 293 cell line lysates transiently transfected with the ATG7 gene (2ug/lane). TOP RIGHT: native 293 cell line lysate. BUTTOM: cos7, HEK293, MEF, and Hela cells, left to right respectively. Data courtesy of Drs. Jiefei Geng and Dan Klionsky, University of Michigan. |
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Autophagy APG7L antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
|
|
Product Citations:
|
- Zhen-Bin Ding, et al. Cancer Res., Nov 2008; 68: 9167 - 9175. (Applications: WB)
Data Rewards Program
>
|
|
Application Notes:
|
| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
|
|
Format:
|
|
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
|
|
Storage:
|
|
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
|
|
Precautions:
|
|
This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
|
Order Information
List Price (USA): $235.00/0.1mg
Related Products
Data Rewards Program
