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Catalog #
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Applications:
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Reactivity:
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Clone Name:
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AP2010b
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FC, WB, IF, IHC, E
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H More
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RB1784
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Concentration:
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Size:
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Accession:
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Isotype:
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0.25 mg/ml
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0.1 mg
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NP_006008, O43490
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Rabbit Ig
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human AC133.
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Other Names:
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Prominin-like protein 1, Antigen AC133, CD133 antigen, PROM1, PROML1, CD133
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Application Data:
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Calculated MW:
97201 Da
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The anti-AC133 Pab (Cat. #AP2010b) is used in Western blot to detect AC133 in Y79 cell lysate. Due to protein glycosylation, the target band is around 120kDa. Immunofluorescence data is of Bone Marrow Mononuclear Cells stained with polyclonal CD133. Image courtesy of Rick Cohen from the Coriell Institute for Medical Research (NJ, USA). |
(LEFT)Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AC133 (CD133) antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
(RIGHT)Immunofluorescence analysis of anti-AC133 (CD133) Antibody (C-term) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI. |
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Product Citations:
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Data Rewards Program
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Application Notes:
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| The suggested dilution will be: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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Order Information
List Price (USA): $235.00/0.1mg
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