|
Catalog #
|
Applications:
|
Reactivity:
|
Clone Name:
|
|
AP3000a
|
WB, IHC, E
|
H
|
RB6903
|
|
Concentration:
|
Size:
|
Accession:
|
Isotype:
|
|
0.25 mg/ml
|
0.1 mg
|
NP_006752, P62258
|
Rabbit Ig
|
|
Target/Specificity:
|
|
This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S58 of human 14-3-3.
|
|
Other Names:
|
|
14-3-3 protein epsilon; 14-3-3E; YWHAE;
|
|
Application Data:
|
|
Calculated MW:
29174 Da
|
|
|
|
|
The anti-YWHAE Pab (Cat. #AP3000a) is used in Western blot to detect YWHAE in mouse brain (left) and HL60 (right) tissue lysates. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
|
|
Product Citations:
|
|
Data Rewards Program
>
|
|
Application Notes:
|
| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
|
|
Format:
|
|
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|
|
Storage:
|
|
Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
|
|
Precautions:
|
|
This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
|
Order Information
List Price (USA): $235.00/0.1mg
Related Products
Data Rewards Program
