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Catalog #
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Applications:
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Reactivity:
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Clone Name:
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AP3289g
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WB, IHC, E
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H, (M), (R) More
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RB11404
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Concentration:
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Size:
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Accession:
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Isotype:
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0.25 mg/ml
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0.1 mg
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NP_003152, P23443
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Rabbit Ig
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S424 of human RPS6KB1.
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Other Names:
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Ribosomal protein S6 kinase beta-1; Ribosomal protein S6 kinase I; S6K; S6K1; 70 kDa ribosomal protein S6 kinase 1; p70 S6 kinase alpha; p70(S6K)-alpha; p70-S6K; P70S6K; p70-alpha; RPS6KB1; STK14A
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Application Data:
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Calculated MW:
59140 Da
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(LEFT)Western blot analysis of RPS6KB1 (arrow) using rabbit polyclonal RPS6KB1 Antibody (S424) (RB11404). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RPS6KB1 gene (Lane 2) (Origene Technologies).
(RIGHT) Western blot analysis of Phospho-RPS6KB1-pS424 Antibody (AP3352a) and RPS6KB1 Antibody (S424) (AP3289g) in human TNF alpha activated Hela cell line lysates. RPS6KB1 and Phospho-RPS6KB1 (arrow) was detected using the purified PAb. (lane 1: without TNF alpha; lane 2: TNF alpha activated, Phospho-RPS6KB1-pS424 Antibody (AP3352a); lane 3: TNF alpha activated, RPS6KB1 Antibody (S424) (AP3289g)) |
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with RPS6KB1 Antibody (T421) (Cat.#AP3289g), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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Order Information
List Price (USA): $235.00/0.1mg
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