|
Catalog #
|
Applications:
|
Reactivity:
|
Clone Name:
|
|
AP3291a
|
DB, E
|
H More
|
RB11133
|
|
Concentration:
|
Size:
|
Accession:
|
Isotype:
|
|
0.25 mg/ml
|
0.1 mg
|
NP_002871, P04049
|
Rabbit Ig
|
|
Target/Specificity:
|
|
This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S296 of human RAF1.
|
|
Other Names:
|
|
RAF proto-oncogene serine/threonine-protein kinase; Raf-1; C-RAF; cRaf; RAF1; RAF;
|
|
Application Data:
|
|
Calculated MW:
73052 Da
|
|
|
|
|
Dot blot analysis of Phospho-RAF1-S296 Pab (Cat.AP3291a) on nitrocellulose membrane. Dot blot analysis of Phospho-RAC1-S71 Pab (Cat.AP3290a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibodies working concentration was 0.5ug per ml. |
|
|
Product Citations:
|
|
Data Rewards Program
>
|
|
Application Notes:
|
| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Dot Blot | 1:500 |
|
|
Format:
|
|
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|
|
Storage:
|
|
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
|
|
Precautions:
|
|
This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
|
Order Information
List Price (USA): $235.00/0.1mg
Related Products
Data Rewards Program
