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Catalog #
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Applications:
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Reactivity:
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Clone Name:
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AP6133a
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WB, E, IHC†
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H, M
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RB2060
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Concentration:
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Size:
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Accession:
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Isotype:
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0.25 mg/ml
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0.1 mg
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NP_037504, O70326
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Rabbit Ig
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminus of human Gremlin.
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Other Names:
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Cysteine KNOT superfamily 1, BMP antagonist 1
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Application Data:
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Calculated MW:
20697 Da
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Western blot analysis of anti-Gremlin Pab (Cat. #AP6133a) in mouse liver cell lysate. Gremlin (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. |
Formalin-PBS and Bouin embedded paraffin human renal tissue pretreated with heat induced epitope retrieval Citrate buffer pH 6.0 in microwave. Dilution of primary antibody was 1:50. Detection method was ABC system or Vectastaint Elite VECTOR. Data and protocol courtesy of Dr. Maria E. Burgos of Universidad Austral de Chile. |
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Product Citations:
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- Mezzano, Sergio, et al. Nephrol. Dial. Transplant. 2007 Jul 01;22(7):1882-1890. (Applications: IHC)
- Wordinger, Robert J., et al. Invest. Ophthalmol. Vis. Sci. 2007 Mar 01;48(3):1191-1200. (Applications: WB)
- Frank, Natasha Y., et al. J. Cell Biol. 2006 Oct 09;175(1):99-110. (Applications: IHC)
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
† Application validated by third-party citation.
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long
term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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Order Information
List Price (USA): $235.00/0.1mg
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