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Catalog #
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Applications:
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Reactivity:
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Clone Name:
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AP6939b
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WB, IHC, FC, E
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H
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RB21888
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Concentration:
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Size:
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Accession:
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Isotype:
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0.25 mg/ml
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0.1 mg
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AP_000649.1, P03915
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Rabbit Ig
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human ND5.
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Other Names:
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NADH-ubiquinone oxidoreductase chain 5; NADH dehydrogenase subunit 5; ND5; NADH5; MTND5; MT-ND5
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Application Data:
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Calculated MW:
67027 Da
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Western blot analysis of ND5 Antibody (C-term) (Cat. #AP6939b) in CEM cell line lysates (35ug/lane). ND5 (arrow) was detected using the purified Pab. |
(LEFT) Formalin-fixed and paraffin-embedded human brain tissue reacted with ND5 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
(RIGHT) Flow cytometric analysis of CEM cells using ND5 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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Product Citations:
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Data Rewards Program
>
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
| Flow cytometric | 1:10~50 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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