|
Catalog #
|
Applications:
|
Reactivity:
|
Clone Name:
|
|
AP7087b
|
WB, IP, E
|
H, M More
|
RB5276
|
|
Concentration:
|
Size:
|
Accession:
|
Isotype:
|
|
0.25 mg/ml
|
0.1 mg
|
NP_000779, P27707
|
Rabbit Ig
|
|
Target/Specificity:
|
|
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human DCK.
|
|
Other Names:
|
|
Deoxycytidine kinase; dCK
|
|
Application Data:
|
|
Calculated MW:
30518 Da
|
|
|
|
|
The anti-DCK Pab (Cat. #AP7087b) is used in Western blot to detect DCK in mouse intestine tissue lysate. |
Deoxycytidine kinase(DCK) immunoprecipitated from H460cells with 7.5ug (microgram) of the dCK antibody (AP7087b) using the Pierce classic mammalian IP kit (#45217) reagent as described as manufacturer instructions (lane 1, 3) and Current Protocols in Cell Biology, 1998, 7.2.1-7.2.21. Proteins separated on a 12% SDS gel, transferred to a PVDF membrane and probed with 1:700 dilution of DCK antibody (AP7087b). Bands were detected using enhanced chemiluminescence (SuperSignal West Pico Chemiluminescent Substrate Kit). No specific reagents were employed to remove IgG from immunoprecipitated sample. Data courtesy of Dr. Stacy Shord, University of Illinois, Chicago. |
|
|
Product Citations:
|
- Christine M. Hartford,, et al. Blood, Dec 2008; 10.1182/blood-2008-05-154302. (Applications: WB)(E-pub)
- Lamba, Jatinder K., et al. J. Pharmacol. Exp. Ther. 2007 Dec 01;323(3):935-945.
Data Rewards Program
>
|
|
Application Notes:
|
| The suggested dilution will be: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| IP | 1:500~1:1000 |
|
|
Format:
|
|
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|
|
Storage:
|
|
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
|
|
Precautions:
|
|
This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
|
Order Information
List Price (USA): $235.00/0.1mg
Related Products
Data Rewards Program
