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SPHK1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)


specificationbackgroundprotocolsprintmsds
 
Catalog # Applications: Reactivity: Clone Name:
AP7237c WB, IHC, E, IP H, M, R, Pr RB3621
Concentration: Size: Accession: Isotype:
0.25 mg/ml 0.1 mg NP_892010, Q9NYA1 Rabbit Ig
Target/Specificity:
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the central region of human SPHK1.
Other Names:
Sphingosine kinase 1, SK 1, SPK 1
Application Data:   Calculated MW:   42517 Da
     

The anti-SPHK1 Pab (Cat. #AP7237c) is used in Western blot to detect SPHK1 in mouse kidney tissue lysate.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Product Citations:
  1. You, Bei, et al. Diabetes 2007 May 01;56(5):1445-1453. (Applications: WB)
  2. Sun, Jianxin, et al. Circ. Res. 2006 Sep 01;99(5):468-476. (Applications: IP)

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Application Notes:
The suggested dilution is:
ELISA1:1,000
Western blotting1:100~500
Immunohistochemistry1:50~100

† Application validated by third-party citation.
Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage:
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Precautions:
This product is for research use only. Not for use in diagnostic or therapeutic procedures.

 

 

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in stockList Price (USA): $235.00/0.1mg

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Abgent is the original manufacturer of this product.
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