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Catalog #
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Applications:
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Reactivity:
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Clone Name:
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AP7501a
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WB, IHC, E
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H, (M) More
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RB0913
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Concentration:
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Size:
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Accession:
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Isotype:
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0.25 mg/ml
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0.1 mg
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P28482
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Rabbit Ig
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human ERK2.
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Other Names:
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MAPK1; protein tyrosine kinase ERK2; extracellular signal-regulated kinase 2; mitogen-activated protein kinase 2; ERK-2; MAP kinase 2; MAPK 2; p42-MAPK; ERT1
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Application Data:
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Calculated MW:
41389 Da
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Western blot analysis of anti-ERK2 C-term Pab (Cat. #AP7501a) in UV-treated HL-60 cell lysate. ERK2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. |
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with ERK2 antibody (C-term) (Cat.#AP7501a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Product Citations:
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Data Rewards Program
>
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Application Notes:
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| The suggested dilution will be: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
| Flow cytometry | |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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