Register or Login
All
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
>   home   >    Crown Insignt   >    pUlk1   

pUlk1

Introduction

Autophagy is induced by growth-dependent import of nutrients, such as amino acids and glucose, as well as through several key signaling molecules such as TOR (or mTOR) and AMPK. Amino acids activate the mTOR pathway, in part, by recruiting mTOR complexes to the lysosomal surface through factors such as the Rag GTPases and p62, and through effects on intracellular lysosomal positioning. The AMP-activated protein kinase (AMPK, red hexagon) responds to glucose uptake, and regulates multiple downstream targets that have effects on autophagy, including mTOR, Atg1 (Ulk1 in mammals, blue hexagon) and CDKs (through cyclin kinase inhibitors; CKIs). Ulk1 has been identified as an AMPK substrate phosphorylated at S467, S556, T574, and S637 after phenformin treatment in vitro.

Fluorescent image of U251 MG cells stained with ULK1 (Phospho S556) Antibody #AP3804a. U251 MG cells treated with Chloroquine (50 μM, 16h) were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min) and incubated with ULK1 (Phospho S556) (1:200, 2 h at RT), followed by secondary antibody, conjugated to Alexa Fluor® 488 (green) (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (5.25 μM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue).

Products labeled with a "crown" meet aggressive quality standards. Learn more.
Antibodies




Immunity Source (Host)
Application
Reactivity
Available staus
PULK1 ()