ULK1

Catalog Products

18,000+ products cover the ten top research categories, including cancer, metabolism, cardiovascular, neuroscience, and stem cells. Each antibody is crafted with care according to rigorous protocols for immunogen design and preparation, presentation to host animal, and high-affinity purification against the antigen. Quality is embedded in the production process to assure the best antibody for you.

View All

Current Promotion

Let us know what your are looking for.

Offer valid for U.S. customer only.Customer pays for shipping.

Learn More

Crown Products

Crown antibodies pass additional stringent quality requirements, including extended control sets, uniform results against multiple biologically relevant cell lines and tissues, and function in multiple applications.

View Details

Current Promotion

Let us know what your are looking for.

Offer valid for U.S. customer only.Customer pays for shipping.

Learn More

New Products

Abgent develops 1000+ new products per year. We provide tools for path-breaking research by developing antibodies that detect a comprehensive library of novel and established targets. For established targets we seek to add antibodies that recognize new epitopes, including post-translational modifications such as phosphorylation and methylation. For new targets we consult with leading experts to accelerate development of antibodies that will propel state-of-the-art research in cellular health and disease.

View Details

Current Promotion

Let us know what your are looking for.

Offer valid for U.S. customer only.Customer pays for shipping.

Learn More

Current Promotion

Let us know what your are looking for.

Offer valid for U.S. customer only.Customer pays for shipping.

Learn More

Current Promotion

Let us know what your are looking for.

Offer valid for U.S. customer only.Customer pays for shipping.

Learn More

Research Focus

Greneral Collections

Peptides

SARS Peptides

Individual peptides for SARS-CoV-2 Spike glycoprotein. In order to study the specificity of cellular immune responses against SARS CoV-2 and potential immunity caused by other human Corona Viruses, Abcepta provides Spike peptide individually, as pools and in plate. These peptides can be used for antigen specific T-cell stimulation in T-cell assays or T-cell expansion.

View All

Synthetic Peptides

All peptides are manufactured in the San Diego, California. Custom peptide services include long peptides (>100 aa), cyclic peptides, difficult sequences, fluorescent labels, phospho-peptides and other post-translational modifications.

View All

Blocking Peptides

The majority of Abcepta antibodies are produced using peptide immunogens. These immuno-specific peptides can be used as blocking agents when using the complementary antibodies in a range of applications.

View All

Cell/Tissues/Lysates

Abcepta's portfolio of cell lines, tissues and lysates are drawn from a range of species and immortalized cell lines. All our cell lines and lysates are validated in key applications.

These tissue lysates can be used in applications such as SDS-PAGE and Western blotting. Whole cell lysates can be used as positive controls for applications such as ELISAs, immunoprecipitation (IP) and Western blotting. Obtaining high-quality whole cell lysates can be tedious as well as time consuming. Abcepta offers a comprehensive portfolio of whole cell lysates for research use. Browse our catalog to find the right whole cell lysate for your experiment.

View All

Citations

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

View Details

Submit your citation using an Abcepta antibody to
info@abcepta.com, and receive a free "I Love Antibodies" mug.


	ULK1
Introduction
The first conserved signaling modules encoded by autophagy genes that control the major steps in the process is the Atg1 complex (Atg1-Atg13-Atg17) in yeast and its mammalian counterpart, the UNC-51 like kinase 1 (ULK1) complex, consisting of ULK1, FIP200, mAtg13 and Atg101. This complex is positively regulated by AMPK, a key energy sensor to low energy and regulator of energy homeostasis. Under nutrient starvation, AMPK promotes autophagy by directly activating ULK1 through phosphorylation of Ser317 and Ser777. Conversely, ULK1 complex is negatively regulated by the mTOR through direct phosphorylation of ULK1 at Ser757. Under nutrient rich conditions, mTOR phosphorylates ULK1 and mAtg13, which inhibits ULK1 kinase activity. During starvation, inactive mTOR allows ULK1 to phosphorylate itself, mAtg13 and FIP200, leading to further recruitment of Atg complexes, such as the class III PI3K complex, to initiate autophagy.	Fluorescent image of U251 cells stained with APG1 (ULK1) (Center) antibody. U251 cells treated with Chloroquine (50 µM,16h), were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min) and incubated with AP8104b APG1 (ULK1) (Center) primary antibody (1:100, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). APG1 (ULK1) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

Recombinant Antibody