March/April 2005

Vol1 Issue 1

In This Issue: Review Article - Kinases in Cancer Featured Article - BH3 Domains in Apoptosis Product Update - New Products for Your Research Customer Corner - Request Our Catalog Tech Corner - SDS-PAGE vs. pI, Citations, SUMOplot™ and Web Links Company Info - Nature Top Supplier, Distributors, Soccer Game Upcoming Meetings - EB 2005 in San Diego, AACR 2005 in Anaheim Kinases in Cancer Review Article - The kinase protein family is one of the largest drug target families in the human genome. Recent successful launches of drugs with kinase inhibition as the mode of action demonstrate the effectiveness of this approach. The latest reports of cancer treatment using kinase inhibitors are reviewed in this article. Read Full Article PKC Antibodies Specific for Isoform nu - Abgent has collaborated with researchers at the UCLA School of Medicine to study the application properties for antibodies made against the different isoforms of PKC kinases. Products Cat.# AP7025a and AP7025b are directed against the PKC nu isoform, also called PKD3. Products Cat.# AP7730a and AP7730b are directed against the PKD-C2 isoform, also called PKD2. Review Data The Cancer Kinome™ - The Cancer Kinome is a phylogenetic tree created by Abgent Bioinformatics staff to illustrate the role of kinases in cancer.More BH3 Domains in Apoptosis Featured Article - Intrafamilial and interfamilial Bcl-2 protein contacts regulate key aspects of apoptosis. Corruption of apoptotic instructions is associated with a large subset of human diseases. Understanding regulation of apoptosis is critical to a strategy for pharmaceutical intervention. This article reviews the BH3 domain and its key role in the regulatory function of Bcl-2 family members. Read Full Article Bcl-2 Antibody Binding is Conformation Dependent - Abgent has collaborated with researchers at The Burnham Institute in La Jolla, CA to investigate the mechanism by which Nur77 targets mitochondria and induces apoptosis. The study utilized Abgent's antibody to the BH3 domain of the Bcl-2 protein (Cat.# AP1303a).Review Data BH3 in Apoptosis - The BH3 in Apoptosis poster charts interactions of apoptotic proteins via BH3 domains surrounding mitochondria. Request a full size poster of the BH3 in Apoptosis for your lab. Product Update New Products Catalog # Product Name Accession # AP6180a MAGEH1 Antibody (N-term) Q9H213 AP7065b MAP4K2 Antibody (Center) M4K2_HUMAN AP7099b PARK8 (LRRK2) Antibody (Center) XP_058513 AP7099a PARK8 (LRRK2) Antibody (N-term) XP_058513 AP7097a PNCK Antibody (C-term) Q6P2M8 AP1202c PRDM2 Antibody NM_012231 AP1225b SUMO3 Antibody (C-term P94) P61956 AP7684b TEK Antibody (N-term) Q969V4 AP1068a UBE2M Antibody (N-term) NP_003960 AP1069a USP21 Antibody (N-term) NP_036607 Hot Products Catalog # Product Name Accession # AP7695a ABL2 Antibody (N-term) P42684 AM1130a AC133 (CD133) Antibody O43490 AP7002a Aurora-A Antibody (N-term) O14965 AP1307a Bid BH3 Domain Antibody NP_001187 AP7209a CAMK2 delta Antibody (C-term) Q13557 AP1032a Dnmt1 Antibody (C-term S1602) NP_001370 AM1009a GFP Tag Antibody AM1008a HA Tag Antibody AP1101b HDAC1 Antibody (N-term) NP_004955 AP7507a JNK3 Antibody (N-term) P53779 AP1312a Mcl-1 BH3 Domain Antibody NP_068779 AP8054b PERK Antibody (C-term) Q9NZJ5 AP7932a PIM1 Antibody (C-term) P11309 AP1201a PRDM1 (BLIMP1) Antibody NP_001189 AP7674c RON Antibody Q04912 AM1200a SUMO1 Monoclonal Antibody AAC50996 AM1101a VEGF4 Antibody NP_004460

Check out our product lines for protein modification research: Phosphorylation SUMOylation Ubiquitination Methylation Acetylation Glycosylation Custom Services Newsletter Sign-Up To continue receiving The Translation Post Subscribe Here Customer Corner Request Abgent 2004-05 Catalog and Technical Reference Tech Corner Q: Why does my protein migrate by SDS-PAGE at an apparent MW different from the calculated MW? A: Check the pI of your protein. Very low or high pI proteins do not bind as well to SDS, and therefore the migration rate is influenced by charge and potential secondary structure in addition to MW. Post-translational modifications such as glycosylation, sumoylation, and hyperphosphorylation can also alter migration rate. Product Application: Check out our latest product citations. Tip: Can't find an antibody in the Abgent catalog for your target of interest? Check out our Name Resolver index on page 281 for a crossindexed list of other names used in the literature. Bioinformatic Tools: Use SUMOplot™ to predict sumoylation sites in your protein sequence. Helpful Web Links: Visit web sites with proteomics analysis tools, protein databases, or for the latest info on pharmaceutical targets in clinical trials. Here Company Info Nature and The Scientist select Abgent among top suppliers: In 2004 issues of Nature (vol.428 No6979 p232) and The Scientist (vol.18 No21 p29), the two periodicals ranked Abgent among the top suppliers for proteomics and phosphorylation reagents. Abgent appoints three new distributors in Europe: AnAdrasis in Greece, BARIA in Czech Republic and Interchim in France. Abgent's current South Korean distributor, DiNonA, has renamed its Marketing & Sales division as ResearchNET, a well known distributor of immunology products merged with DiNonA in 2001. Interchim -France AnAdrasis - Greece BARIA - Czech Republic View All Abgent Distributors Philanthropia: Abgent supported the Christmas 5-a-side football match, Staff vs. Students at the Biomedical Research Centre, Sheffield Hallam University, England. Photos and match report Upcoming Meetings Meet Abgent staff and worldwide representatives at the following conferences: Apr 3-5 Experimental Biology, San Diego, CA at Booth #736 Apr 17-20 AACR, Anaheim, CA at Booth #1741 Worldwide Meetings Abgent, Inc. 10239 Flanders Court, San Diego, CA 92121 1.888.SEL.PABS 858-622-0099 858-622-0609 fax Email: info@abgent.com