Monoclonal Antibody Custom Service is ideal for projects requiring the highest specificity and lowest background. Another advantage of custom monoclonal antibodies is that they are highly homogenous and allow for unlimited production with sustained specificity.
We offer three convenient packages for Monoclonal Antibody Custom Service (MACS) in Balb/c mice or rat, as well as antisera production, testing, and purification services.
Monoclonal Antibody Services
EpiShot™ Premiere MACS
Premiere MACS
Modification-
Specific MACS
Antigen
Custom peptide design and synthesis
Customer-supplied antigen (protein or peptide)
HPLC purification (>90%), LC/MS
Procedure
Peptide-KLH conjugation
Immunization of mice/rats (3 animals)
Serum titration
Hybridoma fusion (best animal)
Clone screening
Stabilization of 10 positive clones
500 ml cultures of 2 clones
Purification
Protein G purification
Product
Purified antibody
20-30 mg
20-30 mg
20-30 mg
Hybridomas (2)
Time
22 weeks
24 weeks
Catalog No
Optional services include: ascites production, clone maintenance, isotype determination, testing (WB, IHC, IP) and more.