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Catalog #
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Source:
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Lot #
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Size:
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AM1008a
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Mouse
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SDG050927B;SG071213A
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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12CA5
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Mouse IgG2b
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Applications:
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Reactivity:
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MW (kDa):
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WB, E, ChIP†, IP†
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N/A
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Target/Specificity:
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KLH conjugated synthetic peptide encoding HA tag (YPYDVPDYA) was used as antigen.
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Other Names:
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Tag from influenza hemagglutinin protein
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Application Data:
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(TOP)HA-tagged recombinant protein was expressed in COS7 cells. 15 ug of cell lysate was loaded onto a gel to test the HA tag antibody (AM1008a).
(Buttom) Western blot analysis of anti-HA Tag Antibody (SG071213A) in 12tag recombinant protein. 12tag recombinant protein was detected using the purified Mab. |
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Background:
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Epitope tags consisting of short sequences recognized by well-characterizated monoclonal antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYA) and Myc Tag (AEEQKLISEEDLLRKRREQLKHKLE), recognized by monoclonal antibody clones 12CA5 and 9E10, respectively, are illustrative examples. Abgent's anti-HA monoclonal antibody (Clone 12CA5) provides a simple solution to detect the expression of an HA-tagged protein in cells.
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Background References:
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- Kolodziej, PA and Young RA. (1991) Methods Enzymol., 194:508-19.
- Sells MA and Chernoff J. (1995) Gene, 152:187-9.
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Product Citations:
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- Guo, Mengjuan, et al. PLANT CELL 2008 Jan 01;20(1):48-58. (Applications: ChIP)
- Chen, Shuzhen, et al. Mol. Pharmacol. 2007 Nov 01;72(5):1269-1279. (Applications: IP)
- Choe, Eun-Ah, et al. J. Neurosci. 2007 Aug 29;27(35):9503-9512. (Applications: WB)
- Hu, Jing, et al. Cancer Res. 2007 Apr 15;67(8):3569-3573. (Applications: WB)
- Mamidipudi, Vidya, et al. Mol. Cell. Biol. 2004 Aug 01;24(15):6788-6798. (Applications: WB)
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Application Notes:
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| ELISA | 1:5,000 |
| Western blotting | 1:1,000 |
† Application validated by third-party citation.
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Format:
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Mouse ascites with 0.09% (W/V) sodium azide.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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