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Catalog #
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Source:
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Lot #
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Size:
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AM1010a
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Mouse
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SDG081217
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0.1mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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1mg/ml
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6AT18
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Mouse IgG1
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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N/A
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1000 Da
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Target/Specificity:
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Purified recombinant HIS-tagged fusion protein and poly-HIS peptide were used to produced this monoclonal antibody.
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Other Names:
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Poly-His, Penta-HIS, Penta-Histidine
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Application Data:
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HIS-tagged recombinant protein was expressed in E. Coli, and used to test the 6xHIS monoclonal antibody (6AT18). |
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Background:
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Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal antibody clone 6AT18 provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. Abgent's anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells.
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Background References:
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- Hochuli E, Doebeli H, and Schacher A. New metal chelate absorbent selective for proteins and peptides containing neighboring histidine residues. J.
- Chromatogr. 1987;411:177-184.
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Product Citations:
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- Unsal-Kacmaz, Keziban, et al. Mol. Cell. Biol. 2007 Apr 15;27(8):3131-3142. (Applications: WB)
- Partch, C.L., et al. Biochemistry 2005 Feb 11.44 (10): 3795-3805. (Applications: WB)
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Application Notes:
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| ELISA | 1:5,000 |
| Western blotting | 1:1,000 |
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Format:
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Monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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