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GST Tag Antibody
Purified Mouse Monoclonal Antibody (Mab)

Catalog # Source: Lot # Size:
AM1011a Mouse SDG060824B 0.1 mg
Concentration: Accession: Clone Name: Isotype:
0.25 mg/ml 9AT106 Mouse IgG1
Applications: Reactivity: MW (kDa):
WB, E N/A 26000 Da
Target/Specificity:
Purified recombinant GST fusion protein was used to produced this monoclonal antibody.
Other Names:
Glutathione S-transferase
Application Data:
GST Tag Antibody

The anti-GST Mab (Cat. #AM1011a) is used in Western blot to detect GST recombinant protein purified from bacterial lysate.
Background:
Glutathione S-transferase (GST) was originally cloned from parasite Schistosoma japonicum and it is now a widely used protein fusion partner. Vectors containing GST Tag have been developed for both prokaryotic and eukaryotic systems. The GST fusion proteins are easily purified from cell lysates by affinity chromatography using Glutathione Sepharose 4B to elute out the GST fusion protein from the column with a denaturing form of glutathione. Using the Abgent anti-GST antibody provides a simple solution to detect the expression of GST fusion proteins in cells.
Background References:
  1. Smith, D.B. and Johnson, K.S., (1988). Gene 67, 31.
  2. Parker, M.W. et al., (1990) J. Mol. Biol. 213, 221.
  3. Toye, B. et al., (1990) Infect. Immun. 58, 3909.
  4. Guan, K.L. and Dixon, J.E. (1991) Anal. Biochem. 192, 262–267.
  5. Ji, X. et al., (1992) Biochemistry 31, 10169.
  6. Davis, A.H. et al. (1993) Biotechnology 11,933–936.
  7. Maru, Y. et al., (1996) J. Biol. Chem. 271, 15353.
  8. Yu, J. et al. (1998) Mol. Cell. Biol. 18, 1379–1387.
Product Citations:
Application Notes:
ELISA1:3,000
Western blotting1:2,000
Format:
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage:
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Precautions:
This product is for research use only. Not for use in diagnostic or therapeutic procedures.