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Catalog #
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Source:
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Lot #
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Size:
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AP1012a
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Rabbit
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SH020610K
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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RB0411
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IF, ChIP, E
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N/A
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Target/Specificity:
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KLH conjugated synthetic peptide encoding HA tag (CYPYDVPDYAYPYDVPDYA) was used as antigen.
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Other Names:
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Tag from influenza hemagglutinin protein
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Application Data:
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Western blot analysis of anti-HA tag Pab (Cat. #AP1012a)in HA-tagged recombinant protein bacterial lysate. |
(Top) GTPase activity of dynamin-2 is required for endocytosis of cell-surface tTG. MRC-5 fibroblasts were transiently transfected with either wild-type (wt) or a GTPase-deficient dynamin-2 mutant (K44A) with a hemagglutinin (HA) tag.(Provided by Evgeny A. Zemskov, Irina Mikhailenko:Journal of Cell Science 120, 3188-3199 (2007))
(Bottom) ChIP was performed with 35S:HATCL1 plants using anti-HA antibodies. Rabbit preimmune serum was used as a mock control. Primer sets specific for the first intron or the 3-UTR region of GL1 were used in PCR reactions. ACTIN2 provided a control(Provided by Shucai Wang, Su-Hwan Kwak:Development 134, 3873-3882 (2007)) |
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Background:
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Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYAYPYDVPDYA) is an established example. Abgent's anti-HA polyclonal antibody provides a simple solution to detect the expression of an HA-tagged protein in cells.
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Background References:
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- Kolodziej, PA and Young RA. (1991) Methods Enzymol., 194:508-19.
- Sells MA and Chernoff J. (1995) Gene, 152:187-9.
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Product Citations:
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- Jianjun Guo, et al. Plant Cell Physiol. 2009; 50:1681-1694. (Applications: WB)(E-pub)
- Marco Scarselli, et al. J. Biol. Chem., Feb 2009; 284: 3577 - 3585. (Applications: IF)
- Yidan Lin, et al. Mol. Cancer Ther., Nov 2008; 7: 3556 - 3565. (Applications: WB)
- Wang, Shucai, et al. Development 2007 Nov 01;134(21):3873-3882. (Applications: ChIP)
- Zemskov, Evgeny A., et al. J. Cell Sci. 2007 Sep 15;120(18):3188-3199. (Applications: IF, WB)
- Liu, Xiangguo, et al. Cancer Res. 2006 Dec 01;66(23):11115-11119. (Applications: WB)
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Application Notes:
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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