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Catalog #
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Source:
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Lot #
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Size:
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AP1013a
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Rabbit
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SH020618S
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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RB0493
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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N/A
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Target/Specificity:
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KLH conjugated synthetic peptide (CDYKDDDDKDYKDDDDK) was used as antigen.
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Other Names:
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Tag from influenza hemagglutinin protein
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Application Data:
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Western blot analysis of a DYKDDDDK-tagged recombinant protein (bacterial lysate) using the DYKDDDDK Tag Antibody (Cat. #AP1013a). |
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Background:
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Epitope tags are useful for the labeling and detection of recombinant proteins using western blotting, immunoprecipitation and immunostaining techniques. The eight amino acid DYKDDDDK peptide is an established and multi-functional epitope tag and can be expressed and detected with a recombinant protein as an amino-terminal or carboxy-terminal fusion (1). Abgents DYKDDDDK antibody binds to the same epitope recognized by Sigma's Anti-FLAG® antibodies.
(FLAG® is a registered trademark of Sigma-Aldrich Co., which is not affiliated with Abgent).
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Background References:
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- Chubet RG, Brizzard BL. Vectors for expression and secretion of FLAG epitope-tagged proteins in mammalian cells. Biotechniques 1996;20(1):136-141
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Product Citations:
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Application Notes:
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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