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Autophagy APG12L Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)

Catalog # Source: Lot # Size:
AP1151a Rabbit SH030325F 0.1 mg
Concentration: Accession: Clone Name: Isotype:
0.25 mg/ml NP_004698, O94817 RB2793 Rabbit Ig
Applications: Reactivity: MW (kDa):
IF, E H 15113 Da
Target/Specificity:
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human APG12L.
Other Names:
Autophagy-related protein 12; APG12-like; ATG12; APG12, APG12L
Application Data:
Autophagy APG12L Antibody (N-term)

Mouse leukaemic monocyte macrophage cells treated with vinblastine for 1 hr. APG12L antibody (Cat AP1151a) detected punctuate staining indicative of nascent autophagic vacuole structures. Data courtesy of Dr. Barry Boland, Department of Pharmacology, Oxford University.
Background:
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG12L is the human homolog of yeast APG12, a ubiquitin-activating enzyme E1-like protein essential for the conjugation system that mediates membrane fusion in autophagy.
Background References:
  1. Tanida, I., et al., Biochem. Biophys. Res. Commun. 296(5):1164-1170 (2002).
  2. Tanida, I., et al., J. Biol. Chem. 277(16):13739-13744 (2002).
  3. Mizushima, N., et al., J. Biol. Chem. 273(51):33889-33892 (1998).
  4. Ueno, K., et al., Hum. Genet. 102(1):63-68 (1998).
Product Citations:
Application Notes:
The suggested dilution is:
ELISA1:1,000
Immunofluorescence1:50~100
Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage:
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Precautions:
This product is for research use only. Not for use in diagnostic or therapeutic procedures.