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Catalog #
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Source:
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Lot #
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Size:
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AP1222a
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Rabbit
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SH020708U
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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AAC50996, P63165
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RB0632
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IF, IHC, E
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H
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11557 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human SUMO1.
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Other Names:
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Small ubiquitin-related modifier 1; SUMO-1; Ubiquitin-like protein SMT3C; SMT3 homolog 3; Ubiquitin-homology domain protein PIC1; Ubiquitin-like protein UBL1; GAP modifying protein 1; GMP1; Sentrin; SMT3C; SMT3H3; UBL1
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Application Data:
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TOP: The anti-SUMO1 polyclonal antibody (Cat. #AP1222a) is used in Western blot to detect GST-SUMO1 fusion protein. BOTTOM: COS-7 cells were transfected for 24 hrs with a plasmid expressing FLAG-ERM (left panels) or FLAG-ERM KR12345 (right panels). Untreated (-) and H2O2-treated (+) cells were collected for immunoblot analysis. Top panels: cell lysates probed by western blot (WB) with an anti-ERM antibody. Center panels: cell lysates immunoprecipitated (IP) with an anti-FLAG antibody followed by WB with AP1222a SUMO-1 antibody. Bottom panels: cell lysates immunoprecipitated with an anti-FLAG antibody followed by WB with AP1224a SUMO-2/3 antibody. (*) represents immunoprecipitated ERM-like forms recognized by anti-SUMO antibodies. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Background:
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Covalent modification of target lysines by SUMO (small ubiquitin-like modifier) modulates processes such as protein localization, transcription, nuclear transport, mitosis, DNA replication and repair, signal transduction, and viral reproduction. SUMO does not seem to be involved in protein degradation and may in fact function as an antagonist of ubiquitin in the degradation process. The SUMO family consists of SUMO1 and closely related homologs SUMO2, SUMO3, and SUMO4. Sumoylation has been shown to regulate a wide range of proteins, including MDM2, PIAS, PML, RanGAP1, RanBP2, p53, p73, HIPK2, TEL, c-Jun, Fas, Daxx, TNFRI, Topo-I, Topo-II, PARK2, WRN, Sp100, IkB-alpha, Androgen receptor (AR), GLUT1/4, CaMK, DNMT3B, TDG, HIF1A, CHD3, EXOSC9, RAD51, and viral targets such as CMV-IE1/2, EBV-BZLF1, and HPV/BPV-E1.
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Background References:
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- Yang, S.H., et al., Mol. Cell 13(4):611-617 (2004).
- Bailey, D., et al., J. Biol. Chem. 279(1):692-703 (2004).
- Ling, Y., et al., Nucleic Acids Res. 32(2):598-610 (2004).
- Pountney, D.L., et al., Exp. Neurol. 184(1):436-446 (2003).
- Ohshima, T., et al., J. Biol. Chem. 278(51):50833-50842 (2003).
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Product Citations:
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- Yanisa Laoong-u-thai, et al. Int J Biol Sci. 2009;5(2):205-14. (Applications: WB)(E-pub)
- Rathke, Christina, et al. J. Cell Sci. 2007 May 01;120(9):1689-1700. (Applications: IF)
- Degerny, Cindy, et al. J. Biol. Chem. 2005 Jul 01;280(26):24330-24338. (Applications: WB)
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Application Notes:
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| Suggested Dilution: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
| Immunofluorescence | |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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