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Catalog #
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Source:
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Lot #
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Size:
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AP1449d
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Rabbit
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SH071123J
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_689807, Q7Z699
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RB13254
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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50477 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human SPRED1.
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Other Names:
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Sprouty-related; EVH1 domain-containing protein 1; Spred-1; SPRED1;
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Application Data:
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Western blot analysis of SPRED1 (arrow) using rabbit polyclonal SPRED1 Antibody (N-term) (Cat.#AP1449d). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SPRED1 gene (Lane 2) (Origene Technologies). |
Formalin-fixed and paraffin-embedded human brain tissue reacted with SPRED1 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Background:
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SPRED1 is a tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase.
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Background References:
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- Brems,H., Nat. Genet. 39 (9), 1120-1126 (2007)
- Szafranski,K., Genome Biol. 8 (8), R154 (2007)
- Yoshida,T., Oncogene 25 (45), 6056-6066 (2006)
- King,J.A., Biochem. J. 388 (PT 2), 445-454 (2005)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
| Immunohistochemistry | 1:10~50 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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