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Catalog #
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Source:
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Lot #
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Size:
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AP1461a
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Rabbit
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SH071211N
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_005462, P46926
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RB13401
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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32669 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human GNPDA1.
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Other Names:
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Glucosamine-6-phosphate deaminase; GNPDA; GlcN6P deaminase; Oscillin
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Application Data:
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Western blot analysis of GNPDA1 (arrow) using rabbit polyclonal GNPDA1 Antibody (N-term) (Cat.#AP1461a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GNPDA1 gene (Lane 2) (Origene Technologies). |
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with *GNPDA1 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Background:
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Glucosamine-6-phosphate deaminase (GNPDA) catalyzes the conversion of glucosamine-6-phosphate to fructose-6-phosphate, a reaction that under physiological conditions proceeds to the formation of fructose-6-phosphate. GNPDA is the sole enzyme linking hexosamine systems with glycolytic pathways, and has been proposed to provide a source of energy in the form of phosphosugar derived from the catabolism of hexosamines found in glycoproteins, glycolipids, and sialic acid-containing macromolecules. GNPDA localizes close to the developing acrosome vesicle and in spermatozoa close to the acrosomal region, and may play a role in the acrosome reaction.
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Background References:
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- Arreola,R., FEBS Lett. 551 (1-3), 63-70 (2003)
- Zhang,J., J. Cell. Biochem. 88 (5), 932-940 (2003)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
| Immunohistochemistry | 1:10~50 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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