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Catalog #
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Source:
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Lot #
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Size:
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AP1473b
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Rabbit
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SA071210AG
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_004052, P55289
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RB12870
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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88351 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human CDH12.
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Other Names:
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Brain cadherin; BR-cadherin; N-cadherin 2; Neural type cadherin 2
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Application Data:
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Western blot analysis of CDH12 Antibody (C-term) (Cat.#AP1473b) in 293 cell line lysates (35ug/lane). CDH12(arrow) was detected using the purified Pab (1:60 dilution). |
Formalin-fixed and paraffin-embedded human brain tissue reacted with CDH12 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Background:
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CDH12 is a type II classical cadherin from the cadherin superfamily of integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. This particular cadherin appears to be expressed specifically in the brain and its temporal pattern of expression would be consistent with a role during a critical period of neuronal development, perhaps specifically during synaptogenesis.
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Background References:
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- Shimoyama,Y., Biochem. J. 349 (PT 1), 159-167 (2000)
- Chalmers,I.J., Genomics 57 (1), 160-163 (1999)
- Kremmidiotis,G., Genomics 49 (3), 467-471 (1998)
- Selig,S., Proc. Natl. Acad. Sci. U.S.A. 94 (6), 2398-2403 (1997)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
| Immunohistochemistry | 1:10~50 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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