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Catalog #
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Source:
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Lot #
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Size:
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AP1474a
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Rabbit
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SH071221B
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_000469, P05186
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RB13414
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H, M
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57323 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human ALPL.
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Other Names:
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AP-TNAP; TNSALP; Alkaline phosphatase liver/bone/kidney isozyme
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Application Data:
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Western blot analysis of ALPL Antibody (N-term) (Cat.#AP1474a) in mouse brain tissue lysates (35ug/lane). ALPL(arrow) was detected using the purified Pab (1:60 dilution). |
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Background:
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There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The genes for the first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. This protein is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization, however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to a disorder known as hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms.
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Background References:
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- Panuccio,V., Am. J. Kidney Dis. 50 (6), 1001-1008 (2007)
- Brun-Heath,I., Eur J Med Genet 50 (5), 367-378 (2007)
- So,P.P., J. Rheumatol. 34 (6), 1313-1322 (2007)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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