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Catalog #
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Source:
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Lot #
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Size:
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AP1475a
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Rabbit
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SH070724E
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_036240, Q9ULZ2
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RB11940
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H
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34160 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human STAP1.
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Other Names:
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STAP-1; Stem cell adaptor protein 1; BCR downstream-signaling protein 1; Docking protein BRDG1
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Application Data:
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Western blot analysis of anti-STAP1 Antibody (N-term) (F56) (RB11940) in mouse kidney tissue lysates (35ug/lane). STAP(arrow) was detected using the purified Pab (1:60 dilution). |
Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with STAP1 antibody (N-term)(F56) (Cat.#AP1475a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Background:
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STAP1 functions as a docking protein acting downstream of Tec tyrosine kinase in B cell antigen receptor signaling. The protein is directly phosphorylated by Tec in vitro where it participates in a postive feedback loop, increasing Tec activity.
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Background References:
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- Ma,J., Atherosclerosis 191 (1), 63-72 (2007)
- Beausoleil,S.A., Nat. Biotechnol. 24 (10), 1285-1292 (2006)
- Gstaiger,M., Science 302 (5648), 1208-1212 (2003)
- Minoguchi,M., J. Biol. Chem. 278 (13), 11182-11189 (2003)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western Blotting | 1:50~100 |
| Immunohistochemistry | 1:50 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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