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Catalog #
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Source:
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Lot #
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Size:
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AP1807a
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Rabbit
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SH050527A
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_071933, Q9NT62
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RB7456
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H, B*, M*, R*
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35864 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human APG3L.
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Other Names:
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Autophagy protein 3-like; APG3-like; hApg3; PC3-96 protein; APG3L; ATG3
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Application Data:
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Polyclonal antibody AP1807a was used in Western blot to detect human APG3L in transfected 293 cells. |
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Background:
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Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole).
APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8 (MAP1LC3) to phosphatidylethanolamine (PE). APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3. Formation of the PE conjugate is essential for autophagy.
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Background References:
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- Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005)
- Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005)
- Greenberg JT. Dev Cell. 8(6):799-801. (2005)
- Levine B. Cell. 120(2):159-62. (2005)
- Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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