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Autophagy APG9L1 Antibody (Center)
Purified Rabbit Polyclonal Antibody (Pab)

Catalog # Source: Lot # Size:
AP1814b Rabbit SH050531N 0.1 mg
Concentration: Accession: Clone Name: Isotype:
0.25 mg/ml NP_076990, Q7Z3C6 RB7504 Rabbit Ig
Applications: Reactivity: MW (kDa):
WB, IHC, E H 94466 Da
Target/Specificity:
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide at the Center of human APG9L1.
Other Names:
Autophagy-related protein 9A; APG9-like 1; APG9L1; ATG9A
Application Data:
Autophagy APG9L1 Antibody (Center)

Western blot analysis of anti-APG9L1 Pab (Cat. #AP1814b) in NCI-H460 cell lysate. APG9L1(arrow) was detected using the purified Pab.

 Autophagy APG9L1 Antibody (Center)

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Background:
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). Apg9 plays a direct role in the formation of the cytoplasm to vacuole targeting and autophagic vesicles, possibly serving as a marker for a specialized compartment essential for these vesicle-mediated alternative targeting pathways.
Background References:
  1. Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005)
  2. Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005)
  3. Greenberg JT. Dev Cell. 8(6):799-801. (2005)
  4. Levine B. Cell. 120(2):159-62. (2005)
  5. Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004)
Product Citations:
Application Notes:
The suggested dilution is:
ELISA1:1,000
Western blotting1:100~500
Immunohistochemistry1:50~100
Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage:
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Precautions:
This product is for research use only. Not for use in diagnostic or therapeutic procedures.