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Catalog #
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Source:
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Lot #
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Size:
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AP1818b
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Rabbit
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SH050527I
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_003757, Q14457
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RB7463
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H, M
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51896 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide surrounding amino acid E225 of human BECN1.
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Other Names:
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Beclin-1; APG6; Coiled-coil myosin-like BCL2-interacting protein; Protein GT197; BECN1; GT197; ATG6
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Application Data:
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(LEFT) Western blot analysis of anti-hBECN1-E225 Pab (Cat. #AP1818b) in 293 cell line lysates transiently transfected with the BECN1 gene (2ug/lane). hBECN1-E225(arrow) was detected using the purified Pab (1:60 dilution).
(RIGHT) The anti-BECN1 Pab (Cat. #AP1818b) is used in Western blot to detect BECN1 in, from left to right, A2058, Hela, mouse brain, Y79, and HL60 tissue lysates. BECN1(arrow) was detected using the purified Pab. |
(LEFT)Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Autophagy BECN1 (APG6) antibody (E225) (Cat.#AP1818b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
(RIGHT)Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Background:
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Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole).
Beclin 1 plays a role in two fundamentally important cell biological pathways: autophagy and apoptosis. Beclin 1 is thought to function as a VPS and autophagy protein as part of a complex with Class III PI3 kinase, Vps34.
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Background References:
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- Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005)
- Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005)
- Greenberg JT. Dev Cell. 8(6):799-801. (2005)
- Levine B. Cell. 120(2):159-62. (2005)
- Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004)
- Liang,X.H., et al. J. Virol. 72 (11), 8586-8596 (1998)
- Aita V.M., et al. Genomics 59:59-65(1999).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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