primary antibodies and peptides 10239 Flanders Court, San Diego, CA 92121, USA. sales@abgent.com
Tel: 858.622.0099, 888-735-7227 (US toll free) Fax: 858-622-0609 (US toll free)

Autophagy RGS19 Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)

Catalog # Source: Lot # Size:
AP1820a Rabbit SH050706H 0.1 mg
Concentration: Accession: Clone Name: Isotype:
0.25 mg/ml NP_005864, P49795 RB7576 Rabbit Ig
Applications: Reactivity: MW (kDa):
WB, IHC, E H 24636 Da
Target/Specificity:
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide at the N-term of human RGS19.
Other Names:
Regulator of G-protein signaling 19; RGS19; G-alpha interacting protein; GAIP protein; GS19; GAIP, GNAI3IP
Application Data:
Autophagy RGS19 Antibody (N-term)

Western blot analysis of anti-RGS19 Pab (Cat. #AP1820a) in and Hela cell lysates. RGS19(arrow) was detected using the purified Pab.

 Autophagy RGS19 Antibody (N-term)

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Background:
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). RGS19 enhances the intrinsic GTPase-activating protein activity of the Galphai3 protein, which stimulates autophagy by favoring the GDP-bound form of Galphai3.
Background References:
  1. Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005)
  2. Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005)
  3. Greenberg JT. Dev Cell. 8(6):799-801. (2005)
  4. Levine B. Cell. 120(2):159-62. (2005)
  5. Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004)
  6. de Vries L., et al. PNAS 93:15203-15208(1996)
  7. de Alba E., et al. J. Mol. Biol. 291:927-939(1999)
  8. Wang J., et al. J. Biol. Chem. 273:26014-26025(1998)
  9. Ogier-Denis E., et al. J. Biol. Chem. 275:39090-39095(2000)
Product Citations:
Application Notes:
The suggested dilution is:
ELISA1:1,000
Western blotting1:100~500
Immunohistochemistry1:50~100
Format:
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage:
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Precautions:
This product is for research use only. Not for use in diagnostic or therapeutic procedures.