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Catalog #
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Source:
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Lot #
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Size:
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AP3021a
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Rabbit
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SDP061207B
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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P31749
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RB7002
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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55686 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y326 of human AKT1.
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Other Names:
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RAC-PK-alpha; Protein kinase B; PKB ; C-AKT
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Application Data:
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The anti-AKT1 ab (Cat. #AP3021a) is used in Western blot to detect AKT1 in mouse stomach (left) mouse lung (right) tissue lysates. |
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Background:
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AKT1belongs to the protein kinase C superfamily. This kinase is activated bythe Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis.
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Background References:
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- Isagawa, T., et al., Mol. Carcinog. 43(1):1-12 (2005).
- Beausoleil, S.A., et al., Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135 (2004).
- Bourguignon, L.Y., et al., J. Biol. Chem. 279(28):29654-29669 (2004).
- Owen, D., et al., J. Biol. Chem. 278(50):50578-50587 (2003).
- Takahashi, M., et al., J. Biochem. 133(2):181-187 (2003).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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