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Catalog #
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Source:
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Lot #
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Size:
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AP3025a
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Rabbit
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SDP071030C
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_000475, P05067
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RB6988
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H
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86943 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S198 of human APP.
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Other Names:
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Amyloid beta A4 protein [Precursor]; APP; ABPP; Alzheimer disease amyloid protein; Cerebral vascular amyloid peptide; CVAP; Protease nexin-II; PN-II; APPI; PreA4; APP; A4; AD1;
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Application Data:
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The anti-Phospho-APP-S198 Pab (Cat. #AP3025a) is used in Western blot to detect Phospho-APP-S198 in Jurkat (left) and mouse brain (right) tissue lysates |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
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Background:
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APP is a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy).
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Background References:
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- Blomqvist, M.E., et al., Neurobiol. Aging 26(6):795-802 (2005).
- Yoon, I.S., et al., J. Biol. Chem. 280(20):20140-20147 (2005).
- Chen, M., et al., Biochem. Biophys. Res. Commun. 330(3):714-721 (2005).
- Yamamoto, M., et al., Am. J. Pathol. 166(5):1475-1485 (2005).
- Moir, R.D., et al., J. Biol. Chem. 280(17):17458-17463 (2005).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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