|
Catalog #
|
Source:
|
Lot #
|
Size:
|
|
AP3134a
|
Rabbit
|
SA080310AM
|
0.1 mg
|
|
Concentration:
|
Accession:
|
Clone Name:
|
Isotype:
|
|
0.25 mg/ml
|
NP_000199, P06213
|
RB7155
|
Rabbit Ig
|
|
Applications:
|
Reactivity:
|
MW (kDa):
|
|
IHC, DB, E
|
H
|
156307 Da
|
|
Target/Specificity:
|
|
This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1361 of human INSR.
|
|
Other Names:
|
|
Insulin receptor [Precursor]; IR; CD220 antigen; INSR;
|
|
Application Data:
|
Dot blot analysis of anti-INSR-pY1361 Pab (Cat. #AP3134a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
|
|
Background:
|
|
After removal of the precursor signal peptide, the insulin receptor precursor is post-translationally cleaved into two chains (alpha and beta) that are covalently linked. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake, thereby mediating the metabolic functions of insulin. Binding to insulin stimulates association of the receptor with downstream mediators including IRS1 and phosphatidylinositol 3'-kinase (PI3K). This protein can activate PI3K either directly by binding to the p85 regulatory subunit, or indirectly via IRS1.
|
|
Background References:
|
- Nakamaru, K., et al., Biochem. Biophys. Res. Commun. 328(2):449-454 (2005).
- Diaz, E., et al., Clin. Biochem. 38(3):243-247 (2005).
- McGettrick, A.J., et al., J. Biol. Chem. 280(8):6441-6446 (2005).
- Schmitt, T.L., et al., J. Biol. Chem. 280(5):3795-3801 (2005).
- Denley, A., et al., Mol. Endocrinol. 18(10):2502-2512 (2004).
|
|
Product Citations:
|
|
|
|
Application Notes:
|
| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Dot BLot | 1:500 |
| Immunohistochemistry | 1:50~100 |
|
|
Format:
|
|
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|
|
Storage:
|
|
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
|
|
Precautions:
|
|
This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
|
