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Catalog #
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Source:
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Lot #
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Size:
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AP3139a
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Rabbit
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SA060811A
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_001560, P51617
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RB7163
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H
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76537 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S376 of human IRAK1.
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Other Names:
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Interleukin-1 receptor-associated kinase 1; IRAK-1; IRAK1; IRAK;
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Application Data:
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The anti-Phospho-IRAK1-S376 Pab (Cat. #AP3139a) is used in Western blot to detect Phospho-IRAK1-S376 in mouse kidney tissue lysate |
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Phospho-IRAK1-S376 Antibody(Cat.#AP3139a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Background:
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IRAK1 binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1. This protein is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B.
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Background References:
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- De Nardo, D., et al., J. Biol. Chem. 280(11):9813-9822 (2005).
- Siedlar, M., et al., Int. J. Cancer 114(1):144-152 (2005).
- Huang, Y., et al., J. Biol. Chem. 279(49):51697-51703 (2004).
- Noubir, S., et al., J. Biol. Chem. 279(24):25189-25195 (2004).
- Cuschieri, J., et al., Shock 21(2):182-188 (2004).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:100~500 |
| Immunohistochemistry | 1:10~50 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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