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Catalog #
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Source:
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Lot #
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Size:
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AP6308a
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Rabbit
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SH071205I
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_066924, O95832
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RB13130
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H
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22613 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Loop1 region of human CLDN1.
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Other Names:
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Claudin-1; Senescence-associated epithelial membrane protein; CLD1; SEMP1; ILVASC
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Application Data:
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Western blot analysis of CLDN1 Antibody (Loop1) (Cat.#AP6308a) in Jurkat cell line lysates (35ug/lane). CLDN1 (arrow) was detected using the purified Pab (1:60 dilution). |
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with hCLDN1-Loop1 (Cat.#AP6308a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Background:
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Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. CLDN1, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome.
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Background References:
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- Kinugasa,T., Anticancer Res. 27 (6A), 3729-3734 (2007)
- Paschoud,S., Mod. Pathol. 20 (9), 947-954 (2007)
- Morohashi,S., Int. J. Mol. Med. 20 (2), 139-143 (2007)
- Krajewska,M., Prostate 67 (9), 907-910 (2007)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
| Immunohistochemistry | 1:10~50 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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