|
Catalog #
|
Source:
|
Lot #
|
Size:
|
|
AP6370b
|
Rabbit
|
SH071204B
|
0.1 mg
|
|
Concentration:
|
Accession:
|
Clone Name:
|
Isotype:
|
|
0.25 mg/ml
|
NP_056956, O15156
|
RB13260
|
Rabbit Ig
|
|
Applications:
|
Reactivity:
|
MW (kDa):
|
|
WB, E
|
H
|
57896 Da
|
|
Target/Specificity:
|
|
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human ZBTB7B.
|
|
Other Names:
|
|
Zinc finger protein 67 homolog; Zfp-67; Zinc finger protein Th-POK; T-helper-inducing POZ/Krueppel-like factor; Krueppel-related zinc finger protein cKrox; hcKrox;Zinc finger and BTB domain-containing protein 15
|
|
Application Data:
|
Western blot analysis of ZBTB7B Antibody (C-term) (Cat.#AP6370b) in 293 cell line lysates (35ug/lane). ZBTB7B (arrow) was detected using the purified Pab (1:60 dilution). |
|
|
Background:
|
|
ZBTB7B is a transcription regulator that acts as a key regulator of lineage commitment of immature T-cell precursors. It is necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. Development of immature T-cell precursors (thymocytes) to either the CD4 helper or CD8 killer T-cell lineages correlates precisely with their T-cell receptor specificity for major histocompatibility complex class II or class I molecules, respectively. ZBTB7B is a transcriptional repressor of the collagen COL1A1 and COL1A2 genes. It may also function as a repressor of fibronectin and possibly other extracellular matrix genes.
|
|
Background References:
|
- Galera,P., Proc. Natl. Acad. Sci. U.S.A. 91 (20), 9372-9376 (1994)
- Widom,R.L., Gene 198 (1-2), 407-420 (1997)
- Heegaard,A.M., J. Bone Miner. Res. 12 (12), 2050-2060 (1997)
- Widom,R.L., Matrix Biol. 20 (7), 451-462 (2001)
|
|
Product Citations:
|
|
|
|
Application Notes:
|
| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
|
|
Format:
|
|
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|
|
Storage:
|
|
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
|
|
Precautions:
|
|
This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
|
