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Catalog #
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Source:
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Lot #
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Size:
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AP7041c
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Rabbit
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SA071029AF
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_002603, Q16654
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RB12836
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, IHC, E
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H
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46469 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human PDK4.
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Other Names:
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Pyruvate dehydrogenase kinase isoform 4; Pyruvate dehydrogenase kinase isozyme 4, mitochondrial
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Application Data:
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Western blot analysis of PDK4 Antibody (E265) in CEM cell line lysates (35ug/lane). PDK4 (arrow) was detected using the purified Pab (1:60 dilution). |
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PDK4-E265, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Background:
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PDK4 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
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Background References:
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- Rosa, G., et al., Obes. Res. 11(2):176-182 (2003).
- Razeghi, P., et al., Cardiology 97(4):203-209 (2002).
- Rowles, J., et al., J. Biol. Chem. 271(37):22376-22382 (1996).
- Gudi, R., et al., J. Biol. Chem. 270(48):28989-28994 (1995).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
| Immunohistochemistry | 1:10~50 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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