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Catalog #
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Source:
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Lot #
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Size:
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AP7258a
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Rabbit
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SH071211P
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_006273, Q13043
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RB13403
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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55499 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human STK4.
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Other Names:
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STE20-like kinase MST1; MST-1; Mammalian STE20-like protein kinase 1; Serine/threonine-protein kinase Krs-2
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Application Data:
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(Left)"Western blot analysis of STK4 (arrow) using rabbit polyclonal STK4 Antibody (N-term) (Cat.#AP7258a).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the STK4 gene (Lane 2) (Origene Technologies)."
(Right)Western blot analysis of STK4 Antibody (N-term) (Cat.#AP7258a) in Jurkat cell line lysates (35ug/lane). STK4 (arrow) was detected using the purified Pab (1:60 dilution). |
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Background:
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STK4 is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. This protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and this protein may induce the chromatin condensation observed in this process.
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Background References:
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- Cinar,B., EMBO J. 26 (21), 4523-4534 (2007)
- Jang,S.W., J. Biol. Chem. 282 (42), 30836-30844 (2007)
- Seidel,C., Mol. Carcinog. 46 (10), 865-871 (2007)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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