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Catalog #
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Source:
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Lot #
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Size:
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AP7826d
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Rabbit
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SH071205D
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_004604, P21980
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RB13081
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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77198 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human TGM2.
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Other Names:
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Tissue transglutaminase; TGase C; TGC; TG(C); Transglutaminase-2; TGase-H
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Application Data:
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Western blot analysis of TGM2 Antibody (Center K444) (Cat.#AP7826d) in K562 cell line lysates (35ug/lane). TGM2 (arrow) was detected using the purified Pab (1:60 dilution). |
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with *TGM2 antibody (Center K444), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Background:
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Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. TGM2 acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. It has been identified as the autoantigen implicated in celiac disease.
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Background References:
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- Porzio,O., Hum. Mutat. 28 (11), 1150 (2007)
- Parsons,A.C., Am J Dermatopathol 29 (5), 433-436 (2007)
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
| Immunohistochemistry | 1:10~50 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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