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Catalog #
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Source:
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Lot #
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Size:
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AP8028c
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Rabbit
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SA071206AD
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0.1 mg
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Concentration:
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Accession:
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Clone Name:
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Isotype:
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0.25 mg/ml
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NP_005018, O00459
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RB13091
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Rabbit Ig
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Applications:
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Reactivity:
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MW (kDa):
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WB, E
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H
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81624 Da
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Target/Specificity:
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This antibody is generated from rabbits immunized with a peptide corresponding to amino acid residues surrounding Y467 of human PIK3R2.
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Other Names:
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Phosphatidylinositol 3-kinase regulatory subunit beta; PI3-kinase p85 subunit beta; PtdIns-3-kinase p85-beta; PIK3R2;
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Application Data:
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Western blot analysis of PIK3R2 (arrow) using rabbit polyclonal PIK3R2 Antibody (Y467) (RB13091). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIK3R2 gene (Lane 2) (Origene Technologies). |
Formalin-fixed and paraffin-embedded human brain tissue reacted with PIK3R2-pY467, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Background:
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Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
PIK3R2 binds to activated Protein Tyrosine Kinases, which are phosphorylated, through its SH2 domain, and acts as an adaptor, mediating the association of the P110 catalytic unit to the plasma membrane.
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Background References:
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- Khan, N.A., et al., J. Neurovirol. 9(6):584-593 (2003).
- Deregibus, M.C., et al., J. Biol. Chem. 277(28):25195-25202 (2002).
- Cook, J.A., et al., J. Immunol. 169(1):254-260 (2002).
- Park, I.W., et al., Blood 97(2):352-358 (2001).
- Zauli, G., et al., FASEB J. 15(2):483-491 (2001).
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Product Citations:
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Application Notes:
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| The suggested dilution is: | |
| ELISA | 1:1,000 |
| Western blotting | 1:50~100 |
| Immunohistochemistry | 1:10~50 |
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Format:
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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Storage:
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Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
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Precautions:
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This product is for research use only. Not for use in diagnostic
or therapeutic procedures.
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