|Application ||WB, IHC-F|
|Calculated MW||21970 Da|
|Purification||Antisera to Human AES (CT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.|
|Immunogen||A 16 amino acid peptide located near human AES carboxy-terminus.|
|Shelf Life||18 months from the date of despatch.|
|Other Names||Amino-terminal enhancer of split, Amino enhancer of split, Gp130-associated protein GAM, Grg-5, Groucho-related protein 5, Protein ESP1, Protein GRG, AES, GRG, GRG5|
|Target/Specificity||Rabbit anti-Human AES1 (C-terminal) antibody recognizes an epitope within the C-Terminal region (CT) of the 197-amino acid protein AES (amino-Terminal enhancer of split), a member of the Groucho/transducin-like enhancer of split (TLE) family of transcriptional regulators, homologous to the amino-Terminal domain of Drosophila Groucho protein.The detachment of integrin from the extracellular matrix (ECM), results in loss of cell adhesion and cell death, termed anoikis. This type of apoptosis is controlled by integrin-mediated signaling pathways and that the mitochondrial protein Bit1 (Bcl-2 inhibitor of transcription), released into the cytoplasm during apoptosis, forms a complex with AES, to induce cell death. The effects of the interaction between Bit1 and AES can be counteracted by the attachment of cells to fibronectin through integrin α5β1.|
|Preservative & Stabilisers||0.02% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-AES (C-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
1. Miyasaka, H. et al. (1993) Molecular cloning and expression of mouse and human cDNA encoding AES and ESG proteins with strong similarity to Drosophila enhancer of split groucho protein.Eur. J. Biochem. 216: 343-352.2. Jan, Y. et al. (2004) A mitochondrial protein, Bit1, mediates apoptosis regulated by integrins and Groucho/TLE corepressors.Cell. 116: 751-762.3. Tetsuka, T. et al. (2000) Inhibition of nuclear factor-kappaB-mediated transcription by association with the amino-terminal enhancer of split, a Groucho-related protein lacking WD40 repeats*.J. Biol. Chem. 275: 4383-4390.
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