|Application ||WB, IHC-F|
|Calculated MW||109735 Da|
|Purification||Antisera to IRE1p were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.|
|Immunogen||A 16 amino acid peptide near the carboxy terminus of human IRE1p.|
|Shelf Life||18 months from the date of despatch.|
|Other Names||Serine/threonine-protein kinase/endoribonuclease IRE1, Endoplasmic reticulum-to-nucleus signaling 1, Inositol-requiring protein 1, hIRE1p, Ire1-alpha, IRE1a, Serine/threonine-protein kinase, 220.127.116.11, Endoribonuclease, 3.1.26.-, ERN1 (HGNC:3449)|
|Target/Specificity||Rabbit anri IRE1p antibody recognizes the C-terminus of inositol-requiring kinase 1 precursor (IRE1p), a 108 kDa serine-threonine kinase/endoribonuclease also known as ERN1. It is an endoplasmic reticulum (ER) transmembrane protein, normally bound to the ER molecular chaperones GRP78 and GRP94. At times of ER stress, due to accumulation of unfolded proteins, IRE1p becomes dissociated from the chaperones, which allows it to activate the un-folded protein transcriptional response (UPR). In the mammalian genome, there are two homologs Ire1alpha and Ire1beta. Ire1alpha is expressed in all cells and tissues, Ire1beta expression is primarily restricted to intestinal epithelial cells.|
|Preservative & Stabilisers||0.02% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-IRE1p (C-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Zhang, K. and Kaufman, R. (2004) Signaling the Unfolded Protein Response from the Endoplasmic Reticulum.J. Biol. Chem. 279: 25935-25938.2. Schroder, M. et al. (2003) IRE1-and HAC1 -independent transcriptional regulation in the unfolded protein response of yeast.Mol. Microbiol. 49: 591-606.3. Tirasophon, W. et al. (1998) A stress response pathway from the endoplasmic reticulum to the nucleus requires a novel bifunctional protein kinase/endoribonuclease (Ire1p) in mammalian cells.Genes Dev. 12: 1812-1824.
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