|Application ||WB, IHC-F|
|Calculated MW||115283 Da|
|Purification||Antisera to NOD2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.|
|Immunogen||14 amino acids at carboxy terminus of human NOD2.|
|Shelf Life||18 months from the date of despatch.|
|Other Names||Nucleotide-binding oligomerization domain-containing protein 2, Caspase recruitment domain-containing protein 15, Inflammatory bowel disease protein 1, NOD2, CARD15, IBD1|
|Target/Specificity||Rabbit anti-Human NODs antibody recognizes NOD2, an intracellular protein of ~100 kDa, which belongs to the NOD (nucleotide binding oligomerisation domain) family. The NOD family comprises at present more than 20 different mammalian NOD-LRR (leucine-rich repeat) proteins, which are involved in the regulation of apoptosis and the immune response. NOD2 has been shown to be constitutively or inducibly expressed in monocytes, macrophages, T and B cells, dendritic cells, as well as intestinal epithelial cells. A specific motif of peptidoglycan (PGN), muramyl dipeptide (MDP), has been identified as the sole lNOD2 and Toll-like receptors (TLR) are emerging as key mediators of innate host defence in the intestinal mucosa, crucially involved in maintaining mucosal and commensal homeostasis.Mutations in the gene encoding NOD2 are a major susceptibility factor underlying a significant subgroup of patients with Inflammatory bowel disease 1 (IBD1), a disease characterized by chronic relapsing inflammation of the gastrointestinal tract, subdivided into Crohn disease and ulcerative colitis phenotypes (Hugotet al.2001). Mutations can also lead to development of Blau syndrome (BLAUS) a rare autosomal dominant condition demonstrating early onset granulomatous arthritis, skin rash and uveitis (Parkhouseet al.2014). Additionally mutations in this gene can lead to development of Early onset sarcoidosis (EOS) characterized by the formation of immune granulomas in affected organs, most often the lungs and lymphatics but often other organs can be involved (Okadaet al.2009).|
|Preservative & Stabilisers||0.02% Sodium Azide (NaN3)|
|Storage||Store at +4℃ or at -20 ℃.|
|Precautions||Anti-Human NOD2 (C-Terminal) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
1. Inohara, N. & Nuñez, G. (2003) NODs: intracellular proteins involved in inflammation and apoptosis.Nat Rev Immunol. 3 (5): 371-82.2. Cario, E. (2005) Bacterial interactions with cells of the intestinal mucosa: Toll-like receptors and NOD2.Gut. 54 (8): 1182-93.3. Girardin, S.E. et al. (2003) Nod2 is a general sensor of peptidoglycan through muramyl dipeptide (MDP) detection.J Biol Chem. 278 (11): 8869-72.
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