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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Anti-Human CD286 Antibody    

Anti-Human CD286 Antibody

Rabbit Anti-Human Polyclonal Antibody

  • WB - Anti-Human CD286 Antibody  ABD10013
    Western blot analysis of whole cell lysate from Jurkat human T-cell leukemia cells probed with Rabbit anti CD286 at 0.5(A), 1(B) and 2(C) ug/ml
  • ICC - Anti-Human CD286 Antibody  ABD10013
    Immunocytochemical staining of Jurkat cells with Rabbit anti Human CD286
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9Y2C9
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Polyclonal IgG
Calculated MW 91880 Da
Additional Information
Purification Antisera to human CD286 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Immunogen A synthetic peptide corresponding to a 13 amino acid sequence located near the centre of human CD286.
Shelf Life 18 months from the date of despatch.
Gene ID 10333
Other Names Toll-like receptor 6, CD286, TLR6
Target/Specificity Rabbit anti-Human CD286 antibody recognizes human CD286, also known as Toll-like receptor 6 (TLR6), a member of the evolutionarily conserved toll-like receptor family (TLRs) and close homologue of CD281 (TLR1), that is expressed by monocytes, CD11c+ immature dendritic cells and endothelial cells of skin microvessels.TLRs play an important role in the recognition of invading microbial pathogens during innate immunity, acting through adaptor molecules such as MyD88 and TRAF6. CD286 acts via a cytokine signaling pathway to induce the activation of NF-kappaB, through the formation of dimers with CD282 (TLR2) which coordinate macrophage activation by Gram-positive bacteria and zymosan. CD286/CD282 dimers are also involved in apoptotic responses through recognition of mycoplasmal lipoproteins.Rabbit anti-Human CD286 antibody does not recognize CD281 (TLR1). Rabbit anti-Human CD286 antibody has been shown as suitable for use in IHC on Jurkat cell cytospins at 2ug/ml.
Preservative & Stabilisers 0.02% Sodium Azide (NaN3)
Storage Store at +4℃ or at -20 ℃.
PrecautionsAnti-Human CD286 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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1. Into, T. et al. (2004) Stimulation of human Toll-like receptor (TLR) 2 and TLR6 with membrane lipoproteins of Mycoplasma fermentans induces apoptotic cell death after NF-kappa B activation.
Cell Microbiol. 6 (2): 187-99.2. Ozinsky, A. et al. (2000) The repertoire for pattern recognition of pathogens by the innate immune system is defined by cooperation between toll-like receptors.
Proc Natl Acad Sci U S A. 97 (25): 13766-71.

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Cat# ABD10013
(40 western blots)
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